The injured, some of whom are in a critical condition, have been sent to a Saifai hospital in Etawah for treatment.
Prime Minister Narendra Modi condoled the death of people in the accident and hoped for fast recovery of those injured.
"The road accident on the Agra-Lucknow Expressway in Uttar Pradesh has caused deep grief. Many passengers have lost their lives ... My condolences to the families of the victims in this hour of grief. I wish those injured recover soon," the Prime Minister's Office said in a tweet quoting Modi.
Uttar Pradesh Chief Minister Yogi Adityanath has directed the district magistrate and other senior officials to reach the accident site and oversee the relief work.
He also issued directives for proper treatment of the injured, a statement issued by the state government said.