Rajiv Kochhar was questioned for the fourth consecutive day on Sunday while Pungalia was examined for the second consecutive day in connection with the case.
CBI, however, questioned Nayak first time in the case to probe his role in offering consultancy services if any to Videocon Group being investigated by the CBI.
Pungalia was also asked over the issue as he was previously an employee of Videocon Group and used to offer consultancy services to the firm.
Nayak and Pungalia were confronted during the questioning, said sources in the CBI.
According to the agency officials, the duo was also asked about the role of Rajiv's Singapore-based company Avista Advisory in the restructuring of loan.
They were also asked about the help Pungalia extended to Videocon in securing the loan from the ICICI Bank, which was part of a Rs 400 billion credit given by a consortium of 20 banks, led by the State Bank of India, to the Videocon Group.
The CBI on Thursday and Friday, Saturday, too, questioned Rajiv Kochhar for hours in connection with the case.
He was on Thursday stopped at Mumbai airport by immigration authorities around 11 a.m. when he was about to leave for Singapore. Later, he was handed over to a CBI which is questioning him as part of its preliminary probe against his brother Deepak Kochhar and Dhoot.
The agency had registered a preliminary inquiry against Deepak Kochhar, Videocon Group officials and others to determine any wrongdoing or otherwise in the sanction of loan to the Group by the ICICI Bank as part of the consortium of banks in 2012.
Chanda Kochhar, who is facing questions of conflict of interest in the case, has not been named in the preliminary inquiry, which was registered after news reports raised questions about the Videocon Chairman giving loan of Rs 640 million to a firm he had jointly promoted with Deepak Kochhar, six months after his group got the Rs 32.50 billion loan.
Sources said Deepak Kochhar and Dhoot could also be called for questioning after examination of documents of the loan and statements.